Reactividad glial en retina lesionada de rata

Monosodium glutamate (MSG) administered to neonatal rats causes a retinal lesion described in literature. There is no agreement in long term evolution of the injury.

We ask if after the neurotoxic retina injury due to MSG, the surviving cells have capacity to proliferate and repairing the damage that were caused in neonatal retina rats. In adult retina rats, we want to determinate the moment of internal blood- retinal barrier (IBRB) maturation, to describe the type of the retinal lesion caused after intravitreal MSG, N-methyl-D-aspartate (NMDA) and kainate, to characterise the response of macroglia and microglia, and to determinate if there is cell proliferation for repairing the damage tissue. Immunhistochemical techniques were made, and the histological sections subsequently were photographed.

The conclusions of the study are: 1/ In neonatal rats we cannot prove the process of regeneration; 2/ The IBRB is well established at 10 days old; 3/ Intravitreal MSG, kainate and NMDA causes a retinal lesion, especially in inner layers. Kainate causes more severe injury. We observe activation and proliferations of microglial cells, (with kainate is more striking), activation of Müller cells with increase of glutamine synthetasa inmunoreactivity. We differentiate also astrocytosis after MSG and kainate, nor NMDA. 4/ In adult rats we neither can prove the process of regeneration.